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BIST™

BIST™ is a brand new LC separation technique developed by SIELC Technologies. The goal of BIST™ is simple – expand the scope of HPLC applications.

SIELC Technologies’ BIST™ is a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns currently on the market! In short, BIST™ is complex chromatography without the hassle.

Bridge Ion Separation Technology™

BIST™ provides an additional separation tool to a whole class of organic and inorganic charged molecules. BIST™ columns, simple mobile phases, and an array of applications provide a whole new continent on the chromatography map.

Download brochure about BIST™ (pdf)
BIST™ mode is useful for separation of neutral hydrophilic, charged hydrophobic and charged hydrophilic, multi-charged hydrophobic and multi-charged hydrophilic compounds.

Analyze Ions Differently

When doubly-charged ions are present in the mobile phase (MP), the surface of the stationary phase can switch its polarity. For example: if the surface is positively-charged and the doubly-charged ions in the mobile phase are sulfate ions with a minus 2 charge (from ionized sulfuric acid), then the surface become negatively-charged.

BIST™ stationery phase with positively charged surface with negative net charge
BIST™ stationery phase with positively charged surface with positive net charge

However, this can occur only when the mobile phase has a relatively low concentration of water. When water is the main component of the mobile phase, it forms a solvation shell around each ion and prevents the surface charge from switching.

The same surface switch is observed when a negatively-charged surface interacts with doubly-charged positive ions such as diamines, and some inorganic ions such as Mg2+ and Ca2+.

BIST™ stationery phase with negatively charged surface with positive net charge