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HPLC Method for Separation of Amines on BIST B and BIST B+ Columns
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies m-Xylylenediamine (MXDA) is a popular curing agent used on epoxy resins. 2,4,6-Tris(dimethylaminomethyl)phenol is another amine-based curing agent used on epoxy resins. Using SIELC’s newly introduced BIST™ method, these amines can be retained and separated on a positively charged, anion-exchange BIST™ B or BIST™ B+ […]
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HPLC Method for Separation of Histidine, Histidine Methyl Ester and Histidine Lauryl Ester on BIST B+ Column
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Histidine is a naturally occurring essential amino acid that the body uses to repair damaged tissue and generate new blood cells. Histidine Methyl Ester is a histidine decarboxylase inhibitor and can be used to synthesize other biological compounds. Histidine Lauryl Ester is another derivative […]
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HPLC Method for Separation of of Amines in Non Aqueous MP on BIST B+ Column
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Dopamine is a key neurotransmitter and medical stimulant used to treat low blood pressure, low heart rate, and heart attacks. m-Xylylenediamine (MXDA) is a popular curing agent used on epoxy resins. 2,4,6-Tris(dimethylaminomethyl)phenol is another amine-based curing agent used on epoxy resins. Using SIELC’s newly introduced […]
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HPLC Method for Analysis of Chromium(III) on BIST B+ Column
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Chromium(III) is a nutrient essential for human metabolism of insulin, sugar, and lipids. Using SIELC’s newly introduced BIST™ method, Cr(III) can be retained on a positively-charged anion-exchange BIST™ B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such […]
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HPLC Method for Analysis of Carnosine (beta-alanyl-L-histidine) on BIST B+ Column
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies High Performance Liquid Chromatography (HPLC) Method for Carnosine (beta-alanyl-L-histidine) Condition Column BIST B+, 4.6×50 mm, 5 µm, 100A Mobile Phase MeCN – 75% Buffer H2SO4 – 0.2% Flow Rate 1.0 ml/min Detection UV 205 nm Peak Retention Time 3.71 min Description Class of Compounds Dipeptide […]
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HPLC Method for Analysis of Fast Blue B Salt on BIST B+ Colum
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Fast Blue B Salt is a multicharged basic dye with a variety of applications in chromatographic testing, including in thin layer chromatography and lipase activity testing. Using SIELC’s newly introduced BIST™ method, Fast Blue B Salt can be retained on a positively-charged anion-exchange BIST™ […]
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HPLC Method for Analysis of DAPI on BIST B+ by SIELC Technologies
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies 4′,6-diamidino-2-phenylindole, also known as DAPI, is a popular fluorescent dye used for DNA staining and fluorescence microscopy. Using SIELC’s newly introduced BIST™ method, DAPI can be retained on a positively-charged anion-exchange BIST™ B+ column. There are two keys to this retention method: 1) a […]
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HPLC Method for Analysis of 3′-Deoxyadenosine and 2′-Deoxyadenosine on BIST B+ Column
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies 3′-Deoxyadenosine (Cordycepin) and 2′-Deoxyadenosine (Deoxyadenosine) are structural isomer derivatives of adenosine. The only structural difference between the two compounds is the replacement of the hydroxyl group (-OH) on either 3’ or 2’ position, respectively. Using SIELC’s newly introduced BIST™ method, 3′-Deoxyadenosine and 2′-Deoxyadenosine can […]
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HPLC Method for Analysis of Deoxyguanosine, Guanine and Guanosine on BIST B+
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Guanine (G) is one of the four nucleobases used to form DNA and is a derivative of purine. It pairs with Cytosine (C) in the double helix of DNA (in RNA it is replaced by Uracil, U). Adding a ribose ring to guanine transforms […]
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Ionic Modifier Effect on Selectivity of Separation of Deoxyguanosine, Guanine and Guanosine on BIST B+
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Guanine (G) is one of the four nucleobases used to form DNA and is a derivative of purine. It pairs with Cytosine (C) in the double helix of DNA (in RNA it is replaced by Uracil, U). Adding a ribose ring to guanine transforms […]