Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

Dopamine is a key neurotransmitter and medical stimulant used to treat low blood pressure, low heart rate, and heart attacks. m-Xylylenediamine (MXDA) is a popular curing agent used on epoxy resins. 2,4,6-Tris(dimethylaminomethyl)phenol is another amine-based curing agent used on epoxy resins. Using SIELC’s newly introduced BIST method, these 3 amines can be retained on a positively-charged anion-exchange BIST B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged analytes to the positively-charged column surface and 2) a mobile phase consisting mostly of less polar organic solvent (such as IPA) to minimize the formation of a solvation layer around the charged analytes. This method uses an entirely non-aqueous mobile phase to drive BIST retention. The gradient starts with a high concentration of the less polar IPA to generate the initial BIST retention and progresses to the more polar MeOH to elute the longer-retaining amines in a reasonable time. Using this new and unique analysis method, these 3 amines can be retained and UV detected at 210 nm.

High Performance Liquid Chromatography (HPLC) Method for Analysis of of Amines in Non Aqueous MP

Condition

Column	BIST™ B+, 4.6×150 mm, 5µm, 100A
Mobile Phase	Gradient IPA/MeOH- 80/20% to 100% MeOH, 5 min , 5 min hold
Buffer	H2SO4 – 0.2%
Flow Rate	1.0 ml/min
Detection	UV 210 nm
Peak Retention Time	3.52 min, 6.12 min, 8.57 min

Description

Class of Compounds	Amines
Analyzing Compounds	Dopamine, m-Xylylenediamine, 2,4,6-Tris(dimethylaminomethyl)phenol