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Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
Dopamine is a key neurotransmitter and medical stimulant used to treat low blood pressure, low heart rate, and heart attacks. m-Xylylenediamine (MXDA) is a popular curing agent used on epoxy resins. 2,4,6-Tris(dimethylaminomethyl)phenol is another amine-based curing agent used on epoxy resins. Using SIELC’s newly introduced BIST method, these 3 amines can be retained on a positively-charged anion-exchange BIST B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged analytes to the positively-charged column surface and 2) a mobile phase consisting mostly of less polar organic solvent (such as IPA) to minimize the formation of a solvation layer around the charged analytes. This method uses an entirely non-aqueous mobile phase to drive BIST retention. The gradient starts with a high concentration of the less polar IPA to generate the initial BIST retention and progresses to the more polar MeOH to elute the longer-retaining amines in a reasonable time. Using this new and unique analysis method, these 3 amines can be retained and UV detected at 210 nm.
|Column||BIST™ B+, 4.6×150 mm, 5µm, 100A|
|Mobile Phase||Gradient IPA/MeOH- 80/20% to 100% MeOH, 5 min , 5 min hold|
|Buffer||H2SO4 – 0.2%|
|Flow Rate||1.0 ml/min|
|Detection||UV 210 nm|
|Peak Retention Time||3.52 min, 6.12 min, 8.57 min|
|Class of Compounds||Amines|
|Analyzing Compounds||Dopamine, m-Xylylenediamine, 2,4,6-Tris(dimethylaminomethyl)phenol|