HPLC Method for Analysis of Chromium(III) on BIST B+ Column

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Chromium(III) on BIST B+ Column
HPLC Method for Analysis of Chromium(III) on BIST B+ by SIELC Technologies

Chromium(III) is a nutrient essential for human metabolism of insulin, sugar, and lipids. Using SIELC’s newly introduced BIST™ method, Cr(III) can be retained on a positively-charged anion-exchange BIST™ B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively charged trivalent Chromium ion to the positively charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Chromium(III) can be separated, retained, and UV detected at 200 nm.

High Performance Liquid Chromatography (HPLC) Method for Chromium(III)


Condition

ColumnBIST B+, 4.6×50 mm, 5µm, 100A
Mobile PhaseGradient MeCN – 60-20%, 10 min
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 200 nm
Peak Retention Time5.71 min
LOD0.4 ppm

Description

Class of CompoundsIon
Analyzing CompoundsChromium(III)