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Join our Bridge Ion Separation Technology project at researchgate.net!
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
Chromium(III) is a nutrient essential for human metabolism of insulin, sugar, and lipids. Using SIELC’s newly introduced BIST™ method, Cr(III) can be retained on a positively-charged anion-exchange BIST™ B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively charged trivalent Chromium ion to the positively charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Chromium(III) can be separated, retained, and UV detected at 200 nm.
Column | BIST B+, 4.6×50 mm, 5µm, 100A |
Mobile Phase | Gradient MeCN – 60-20%, 10 min |
Buffer | H2SO4 – 0.2% |
Flow Rate | 1.0 ml/min |
Detection | UV 200 nm |
Peak Retention Time | 5.71 min |
LOD | 0.4 ppm |
Class of Compounds | Ion |
Analyzing Compounds | Chromium(III) |