Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

Chromium(III) is a nutrient essential for human metabolism of insulin, sugar, and lipids. Using SIELC’s newly introduced BIST™ method, Cr(III) can be retained on a positively-charged anion-exchange BIST™ B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively charged trivalent Chromium ion to the positively charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Chromium(III) can be separated, retained, and UV detected at 200 nm.

High Performance Liquid Chromatography (HPLC) Method for Chromium(III)

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Column	BIST B+, 4.6×50 mm, 5µm, 100A
Mobile Phase	Gradient MeCN – 60-20%, 10 min
Buffer	H2SO4 – 0.2%
Flow Rate	1.0 ml/min
Detection	UV 200 nm
Peak Retention Time	5.71 min
LOD	0.4 ppm

Description

Class of Compounds	Ion
Analyzing Compounds	Chromium(III)