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Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
Chromium(III) is a nutrient essential for human metabolism of insulin, sugar, and lipids. Using SIELC’s newly introduced BIST™ method, Cr(III) can be retained on a positively-charged anion-exchange BIST™ B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively charged trivalent Chromium ion to the positively charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Chromium(III) can be separated, retained, and UV detected at 200 nm.
|Column||BIST B+, 4.6×50 mm, 5µm, 100A|
|Mobile Phase||Gradient MeCN – 60-20%, 10 min|
|Buffer||H2SO4 – 0.2%|
|Flow Rate||1.0 ml/min|
|Detection||UV 200 nm|
|Peak Retention Time||5.71 min|
|Class of Compounds||Ion|