Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

Polyhexanide (polyhexamethylene biguanide, PHMB) has multiple industrial, medical, biological and research applications. It has strong antiseptic properties that make this compound very useful for materials that could be a possible source of bacterial contamination.  Using SIELC’s newly introduced BIST™ method, Polyhexanide (polyhexamethylene biguanide, PHMB) can be retained on a positively-charged anion-exchange BIST™ B+ column. 

There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged analytes to the positively-charged column surface and 2) a mobile phase consisting of a majority of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Utilizing a step gradient to switch to a completely aqueous MP after 2 minutes allows for retention to occur while also preventing the method from being too long. Using this new and unique analysis method, PHMB can be separated, retained, and UV detected at 200 nm.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Polyhexanide (polyhexamethylene biguanide, PHMB)

Condition

Column	BIST™ B+, 4.6×50 mm, 5µm, 100A
Mobile Phase	Step gradient MeCN
Buffer	H2SO4 – 0.2%
Flow Rate	1.0 ml/min
Detection	UV 200 nm

Description

Class of Compounds	Polymers
Analyzing Compounds	Polyhexanide (polyhexamethylene biguanide, PHMB)