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Separation type: Hydrophilic interaction liquid chromatography (HILIC)
Uric acid is the final product of purine metabolism and is commonly found in urine. Using SIELC’s newly introduced BIST™ method, this purine metabolite can be retained and separated from urine and plasma on a positively-charged anion-exchange BIST™ B+ column.
There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged analytes to the positively-charged column surface and 2) a mobile phase consisting of a majority of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Uric Acid can be separated, retained, and UV detected at 260 and 290 nm.
|Column||BIST™ B+, 4.6×150 mm, 5µm, 100A|
|Mobile Phase||MeCN – 85%|
|Buffer||Formic Acid – 0.5%|
|Flow Rate||1.0 ml/min|
|Detection||UV 290 nm|
|Retention time||9.09 min|
|Class of Compounds||Acid|
|Analyzing Compounds||Uric Acid|