HPLC Method for Analysis of Uric Acid in Urine and Human Serum Samples on BIST B+ Column by SIELC Technologies

Separation type: Hydrophilic interaction liquid chromatography (HILIC)

HPLC Method for Analysis of Uric Acid in Urine and Human Serum Samples on BIST B+ Column by SIELC Technologies
HPLC Method for Analysis of Uric Acid in Urine and Human Serum Samples on BIST B+ by SIELC Technologies.

Uric acid is the final product of purine metabolism and is commonly found in urine. Using SIELC’s newly introduced BIST™ method, this purine metabolite can be retained and separated from urine and plasma on a positively-charged anion-exchange BIST™ B+ column. 

There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged analytes to the positively-charged column surface and 2) a mobile phase consisting of a majority of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Uric Acid can be separated, retained, and UV detected at 260 and 290 nm.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Uric Acid in Urine and Human Serum Samples

Condition
ColumnBIST™ B+, 4.6×150 mm, 5µm, 100A
Mobile PhaseMeCN – 85%
BufferFormic Acid – 0.5%
Flow Rate1.0 ml/min
DetectionUV 290 nm
Retention time9.09 min
Description
Class of CompoundsAcid
Analyzing CompoundsUric Acid