HPLC Method for Analysis of Tartrazine on BIST A Column

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Tartrazine on BIST A Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of  Tartrazine

Tartrazine is a popular yellow synthetic dye used in a multitude of food and cosmetic products. Using SIELC’s newly introduced BIST™ method, tartrazine, which ionizes in water, can be retained on a negatively-charged, cation-exchange BIST™ A column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged tartrazine analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. The effect of reducing the solvation layer by increasing the organic component concentration in the mobile phase can be clearly seen above, as can the effect of multi- and singly-charged buffers on retention. Other positively-charged buffers that can generate BIST™ include Calcium acetate and Magnesium acetate. Using this new and unique analysis method, tartrazine can be retained and UV detected at 260 nm.


ColumnBIST™ A, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN
BufferTMDAP formate pH 4.0 – 5,0 mM
Flow Rate1.0 ml/min
Detection260 nm


Class of CompoundsAcid, Dye
Analyzing CompoundsTartrazine