HPLC Method for Analysis of Polyglutamic Acid on BIST A

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Polyglutamic Acid on BIST A column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of  Polyglutamic Acid

Polyglutamic acid (PGA) is a biopolymer consisting of repeating units of Glutamic acid. PGA can be found and used in a wide variety of industries due to its biodegradable, non-toxic properties, including in the food, medical, and wastewater industries. Using SIELC’s newly introduced BIST™ method, PGA can be retained easily on a negatively-charged, cation-exchange BIST™ A column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N’-Dimethylpiperazine (DMP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include TMDAP, Calcium acetate, and Magnesium acetate. Using this new and unique analysis method, PGA can be retained with high selectivity and great peak shape. This method can be detected and is compatible with ELSD, CAD, and Mass Spectrometry (LC-MS).


ColumnBIST™ A, 4.6×50 mm, 5 µm, 100A
Mobile PhaseGradient MeCN
BufferN,N’-Dimethylpiperazine formate pH 4.0
Flow Rate1.0 ml/min
DetectionELSD, 70C


Class of CompoundsAcid, Polymer, Peptide
Analyzing CompoundsPolyglutamic Acid