HPLC Method for Analysis of Neurotransmitters Phenylephrine, Epinephrine and Norepinephrine on BIST™B+ Column

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Neurotransmitters Phenylephrine, Epinephrine and Norepinephrine on BIST™B+ Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Phenylephrine, Epinephrine, Norepinephrine

Epinephrine and norepinephrine are key neurotransmitters and adrenal hormones naturally produced by the body to generate the fight-or-flight response, and synthesized by pharmaceutical companies to treat a host of issues, such as allergic reactions and low blood pressure. Phenylephrine is a related, synthetic compound made to treat low blood pressure, congestion, and hemorrhoids. Using SIELC’s newly introduced BIST™ method, these three similar neurotransmitters, which protonate in water, can be retained on a positively-charged anion-exchange BIST™ B+ column.

There are two keys to this retention method:

1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged amine analytes to the positively-charged column surface and

2) a mobile phase consisting mostly of organic solvent to minimize the formation of a solvation layer around the charged analytes.

Using this new and unique analysis method, Epinephrine, norepinephrine, and phenylephrine can be retained and UV detected at 200 nm.  

Condition

ColumnBIST™ B+, 4.6×150 mm, 5µm, 100A
Mobile PhaseMeCN – 80%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 200nm

Description

Class of CompoundsDrug, Neurotransmitter, Catecholamine
Analyzing Compounds Phenylephrine, Epinephrine, Norepinephrine