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Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
Epinephrine and norepinephrine are key neurotransmitters and adrenal hormones naturally produced by the body to generate the fight-or-flight response, and synthesized by pharmaceutical companies to treat a host of issues, such as allergic reactions and low blood pressure. Phenylephrine is a related, synthetic compound made to treat low blood pressure, congestion, and hemorrhoids. Using SIELC’s newly introduced BIST™ method, these three similar neurotransmitters, which protonate in water, can be retained on a positively-charged anion-exchange BIST™ B+ column.
There are two keys to this retention method:
1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged amine analytes to the positively-charged column surface and
2) a mobile phase consisting mostly of organic solvent to minimize the formation of a solvation layer around the charged analytes.
Using this new and unique analysis method, Epinephrine, norepinephrine, and phenylephrine can be retained and UV detected at 200 nm.
|Column||BIST™ B+, 4.6×150 mm, 5µm, 100A|
|Mobile Phase||MeCN – 80%|
|Buffer||H2SO4 – 0.2%|
|Flow Rate||1.0 ml/min|
|Class of Compounds||Drug, Neurotransmitter, Catecholamine|
|Analyzing Compounds||Phenylephrine, Epinephrine, Norepinephrine|