Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

Epinephrine and norepinephrine are key neurotransmitters and adrenal hormones naturally produced by the body to generate the fight-or-flight response, and synthesized by pharmaceutical companies to treat a host of issues, such as allergic reactions and low blood pressure. Phenylephrine is a related, synthetic compound made to treat low blood pressure, congestion, and hemorrhoids. Using SIELC’s newly introduced BIST™ method, these three similar neurotransmitters, which protonate in water, can be retained on a positively-charged anion-exchange BIST™ B+ column.

There are two keys to this retention method:

1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged amine analytes to the positively-charged column surface and

2) a mobile phase consisting mostly of organic solvent to minimize the formation of a solvation layer around the charged analytes.

Using this new and unique analysis method, Epinephrine, norepinephrine, and phenylephrine can be retained and UV detected at 200 nm.  

BIST B+ Column 4.6x150mm 5um 100A

Condition

Column	BIST™ B+, 4.6×150 mm, 5µm, 100A
Mobile Phase	MeCN – 80%
Buffer	H2SO4 – 0.2%
Flow Rate	1.0 ml/min
Detection	UV 200nm

Description

Class of Compounds	Drug, Neurotransmitter, Catecholamine
Analyzing Compounds	Phenylephrine, Epinephrine, Norepinephrine