Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

2-Naphthalenesulfonic acid and 1,5-Naphthalenedisulfonic acid, also known as Armstrong’s acid, are two similarly-structured compounds. 2-Naphthalenesulfonic acid is commonly used in dye production and 1,5-Naphthalenedisulfonic acid is used in synthesizing the salts of basic drugs and in electrokinetic chromatography. Using SIELC’s newly introduced BIST™ method, these two acids can be retained on a negatively-charged, cation-exchange BIST™ A column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged tartrazine analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, 2-Naphthalenesulfonic acid and 1,5-Naphthalenedisulfonic acid can be retained and UV detected at 270 nm.

BIST A Column 4.6x150mm 5um 100A

Condition

Column	BIST™ A, 4.6×150 mm, 5 µm, 100A
Mobile Phase	MeCN – 80/20%
Buffer	TMDAP formate pH 4.0 – 5,0 mM
Flow Rate	1.0 ml/min
Detection	270 nm

Description

Class of Compounds	Acid, Sulfonic acid
Analyzing Compounds	2 – Naphthalenesulfonic acid and 1,5-Naphthalenedisulfonic acid