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Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
2-Naphthalenesulfonic acid and 1,5-Naphthalenedisulfonic acid, also known as Armstrong’s acid, are two similarly-structured compounds. 2-Naphthalenesulfonic acid is commonly used in dye production and 1,5-Naphthalenedisulfonic acid is used in synthesizing the salts of basic drugs and in electrokinetic chromatography. Using SIELC’s newly introduced BIST™ method, these two acids can be retained on a negatively-charged, cation-exchange BIST™ A column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged tartrazine analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, 2-Naphthalenesulfonic acid and 1,5-Naphthalenedisulfonic acid can be retained and UV detected at 270 nm.
Column | BIST™ A, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN – 80/20% |
Buffer | TMDAP formate pH 4.0 – 5,0 mM |
Flow Rate | 1.0 ml/min |
Detection | 270 nm |
Class of Compounds | Acid, Sulfonic acid |
Analyzing Compounds | 2 – Naphthalenesulfonic acid and 1,5-Naphthalenedisulfonic acid |