Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

The maleate ion from Maleic acid is a popular ingredient as the maleate salt in several different drugs, including Methergine, Pyrilamine, and Carfenazine, among others. Nicotinic acid, also known as Niacin or Vitamin B3, is an essential nutrient for the human body and is sometimes taken as a treatment for high cholesterol. Aconitic acid is a key intermediary in the citric acid cycle, and is also used a flavoring agent and in the production of rubbers and plastics. Fumaric acid is a popular preservative and food additive with a fruit-like taste. Using SIELC’s newly introduced BIST™ method, a mixture of these organic acids can be separated on a negatively-charged, cation-exchange BIST™ A+ column, contrary to conventional chromatographic wisdom. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include Calcium acetate and Magnesium acetate. Using this new and unique analysis method, these organic acids can be separated, retained, and detected through ELSD. This method is also compatible with Mass Spectrometry (LC-MS) and CAD.

BIST A+ Column 4.6x150mm 5um 100A

Condition

Column	BIST™ A+, 4.6×150 mm, 5 µm, 100A
Mobile Phase	MeCN Gradient
Buffer	TMDAP ( N,N,N’,N’-Tetramethyl-1,3-diaminopropane) phosphate  – 5 mM pH 4.0
Flow Rate	1.0 ml/min
Detection	UV 250 nm

Description

Class of Compounds	Acids, Organic acid
Analyzing Compounds	Maleic  acid, Ascorbic acid, Nicotinic acid, Fumaric acid and Oxalic acid