HPLC Method for Analysis of Low MW Polylysine on BIST™ B+ Column

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Low MW  Polylysine on BIST™ B+ Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Polylysine

Polylysine includes a large group of similar polymers with various uses. Some are used as food preservatives, while others are used for drug delivery in pharmaceuticals. Polymers with charged monomeric units, such as polylysine, are often difficult to separate using typical ion-exchange chromatography due to very strong and often irreversible interactions with the oppositely charged column surface. Therefore, an extremely high concentration of the buffer, up to several molar, is usually needed to facilitate an ion-exchange process. This high buffer concentration, however, is not desirable because of the significantly increased viscosity of the mobile phase and the salt formation in the pump components. With BIST™, these polymers can be separated and retained with relatively weak buffers (in the mM regime) and a fairly simple gradient. Using this new and unique analysis method, polylysine can be retained and UV detected at 205 nm.


ColumnBIST™ B+, 4.6×50 mm, 5µm, 100A
Mobile PhaseGradient MeCN
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 205 nm


Class of CompoundsPeptide, Homopolypeptide
Analyzing Compounds Polylysine