HPLC Method for Analysis of Inorganic anions on BIST™A+ Column

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Inorganic anions on BIST™A+ Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Inorganic anions, Chloride, Bromide, Nitrate, Iodide,  Perchlorate

Using SIELC’s newly introduced BIST™ method, a mixture of many different inorganic anions can be separated on a negatively-charged, cation-exchange BIST™ A column, contrary to conventional chromatographic wisdom. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include Calcium acetate and Magnesium acetate. Using this new and unique analysis method, these anions can be separated, retained, and detected through ELSD. This method is also compatible with Mass Spectrometry.


ColumnBIST™ A+, 4.6×50 mm, 5µm, 100A
Mobile PhaseMeCN – 90%
BufferTMDAP ( N,N,N’,N’-Tetramethyl-1,3-diaminopropane) formate – 5 mM pH 4.0
Flow Rate1.0 ml/min
DetectionELSD, 70C


Class of CompoundsAcid, Inorganic anion
Analyzing CompoundsChloride, Bromide, Nitrate, Iodide,  Perchlorate, TFA, Methanesulfonic acid