Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

Heparin is a WHO-recognized essential medicine known for its anticoagulant properties and used to treat heart attacks and angina. Due to its very polar and polymeric nature, separating and quantifying Heparin using traditional HPLC methods can be quite difficult. Using SIELC’s newly introduced BIST™ method, however, Heparin and its salts can be separated easily on a negatively-charged, cation-exchange BIST™ A column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N’-Dimethylpiperazine (DMP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include TMDAP, Calcium acetate, and Magnesium acetate. Using this new and unique analysis method, Heparin and its salts can be separated and retained with high selectivity and great peak shape. This method can be detected and is compatible with ELSD, CAD, and Mass Spectrometry (LC-MS).

BIST A Column 4.6x50mm 5um 100A

Condition

Column	BIST™ A, 4.6×50 mm, 5 µm, 100A
Mobile Phase	Gradient MeCN
Buffer	N,N’-Dimethylpiperazine acetate pH 4.0
Flow Rate	1.0 ml/min
Detection	ELSD, 70C

Description

Class of Compounds	Drug, Glycosaminoglycan
Analyzing Compounds	Heparin