HPLC Method for Analysis of Fast Blue B Salt on BIST B+ Colum

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Fast Blue B Salt on BIST B+ Colum
HPLC Method for Analysis of Fast Blue B Salton BIST B+ by SIELC Technologies.

Fast Blue B Salt is a multicharged basic dye with a variety of applications in chromatographic testing, including in thin layer chromatography and lipase activity testing. Using SIELC’s newly introduced BIST™ method, Fast Blue B Salt can be retained on a positively-charged anion-exchange BIST™ B+ column. 

There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged analytes to the positively-charged column surface and 2) a mobile phase consisting of a majority of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Utilizing a step gradient to switch to a completely aqueous MP after 2 minutes allows for retention to occur while also preventing the method from being too long. Using this new and unique analysis method, Fast Blue B Salt can be separated, retained, and UV detected at 320 nm.

High Performance Liquid Chromatography (HPLC) Method for Analysis of DAPI

Condition
ColumnBIST™ B+, 4.6×150 mm, 5µm, 100A
Mobile PhaseGradient MeCN
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 320 nm
Retention time6.01 min
Description
Class of CompoundsDye
Analyzing CompoundsFast Blue B Salt