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Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
Fast Blue B Salt is a multicharged basic dye with a variety of applications in chromatographic testing, including in thin layer chromatography and lipase activity testing. Using SIELC’s newly introduced BIST™ method, Fast Blue B Salt can be retained on a positively-charged anion-exchange BIST™ B+ column.
There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged analytes to the positively-charged column surface and 2) a mobile phase consisting of a majority of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Utilizing a step gradient to switch to a completely aqueous MP after 2 minutes allows for retention to occur while also preventing the method from being too long. Using this new and unique analysis method, Fast Blue B Salt can be separated, retained, and UV detected at 320 nm.
|Column||BIST™ B+, 4.6×150 mm, 5µm, 100A|
|Mobile Phase||Gradient MeCN|
|Buffer||H2SO4 – 0.2%|
|Flow Rate||1.0 ml/min|
|Detection||UV 320 nm|
|Retention time||6.01 min|
|Class of Compounds||Dye|
|Analyzing Compounds||Fast Blue B Salt|