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Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
3′-Deoxyadenosine (Cordycepin) and 2′-Deoxyadenosine (Deoxyadenosine) are structural isomer derivatives of adenosine. The only structural difference between the two compounds is the replacement of the hydroxyl group (-OH) on either 3’ or 2’ position, respectively. Using SIELC’s newly introduced BIST™ method, 3′-Deoxyadenosine and 2′-Deoxyadenosine can be retained and separated on a positively-charged anion-exchange BIST™ B+ column.
There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Phosphoric acid (H3PO4), which acts as a bridge, linking the positively-charged analytes to the positively-charged column surface and 2) a mobile phase consisting of a majority of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, 3′-Deoxyadenosine and 2′-Deoxyadenosine can be separated, retained, and UV detected at 260 nm.
|Column||BIST™ B+, 4.6×150 mm, 5µm, 100A|
|Mobile Phase||MeCN -85%|
|Flow Rate||1.0 ml/min|
|Detection||UV 260 nm|
|Retention time||8.2, 9.8 min|
|Class of Compounds||Nucleosides|
|Analyzing Compounds||3′-Deoxyadenosine (Cordycepin) and 2′-Deoxyadenosine (Deoxyadenosine)|