Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

3′-Deoxyadenosine (Cordycepin) and 2′-Deoxyadenosine (Deoxyadenosine) are structural isomer derivatives of adenosine. The only structural difference between the two compounds is the replacement of the hydroxyl group (-OH) on either 3’ or 2’ position, respectively. Using SIELC’s newly introduced BIST™ method, 3′-Deoxyadenosine and 2′-Deoxyadenosine can be retained and separated on a positively-charged anion-exchange BIST™ B+ column. 

There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Phosphoric acid (H3PO4), which acts as a bridge, linking the positively-charged analytes to the positively-charged column surface and 2) a mobile phase consisting of a majority of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, 3′-Deoxyadenosine and 2′-Deoxyadenosine can be separated, retained, and UV detected at 260 nm.

High Performance Liquid Chromatography (HPLC) Method for 3′-Deoxyadenosine and 2′-Deoxyadenosine

Condition

Column	BIST™ B+, 4.6×150 mm, 5µm, 100A
Mobile Phase	MeCN -85%
Buffer	H3PO4 -0.2%
Flow Rate	1.0 ml/min
Detection	UV 260 nm
Retention time	8.2, 9.8 min

Description

Class of Compounds	Nucleosides
Analyzing Compounds	3′-Deoxyadenosine (Cordycepin) and 2′-Deoxyadenosine (Deoxyadenosine)