Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

Cytochrome C is a mitochondrial protein that is essential to both the respiratory electron transport chain and the apoptosis cycle of cells. Using SIELC’s newly introduced BIST™ method, this essential protein can be retained on a positively-charged anion-exchange BIST™ B column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged amine analytes to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Cytochrome C can be retained and UV detected at 395 nm.

BIST B+ Column 4.6x150mm 5um 100A

Condition

Column	BIST™ B+, 4.6×150 mm, 5 µm, 100A
Mobile Phase	Gradient MeCN
Buffer	H2SO4 – 0.2%
Flow Rate	1.0 ml/min
Detection	UV 395 nm

Description

Class of Compounds	Protein, Hemeprotein
Analyzing Compounds	Cytochrome C