HPLC Method for Analysis of Cytochrome C on BIST™ B+ Column

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Cytochrome C on BIST™ B+ Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Cytochrome C on a BIST™ B Column

Cytochrome C is a mitochondrial protein that is essential to both the respiratory electron transport chain and the apoptosis cycle of cells. Using SIELC’s newly introduced BIST™ method, this essential protein can be retained on a positively-charged anion-exchange BIST™ B column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged amine analytes to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Cytochrome C can be retained and UV detected at 395 nm.


ColumnBIST™ B+, 4.6×150 mm, 5 µm, 100A
Mobile PhaseGradient MeCN
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 395 nm


Class of CompoundsProtein, Hemeprotein
Analyzing CompoundsCytochrome C