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Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
Cytochrome C is a mitochondrial protein that is essential to both the respiratory electron transport chain and the apoptosis cycle of cells. Using SIELC’s newly introduced BIST™ method, this essential protein can be retained on a positively-charged anion-exchange BIST™ B column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged amine analytes to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Cytochrome C can be retained and UV detected at 395 nm.
|Column||BIST™ B+, 4.6×150 mm, 5 µm, 100A|
|Mobile Phase||Gradient MeCN|
|Buffer||H2SO4 – 0.2%|
|Flow Rate||1.0 ml/min|
|Detection||UV 395 nm|
|Class of Compounds||Protein, Hemeprotein|
|Analyzing Compounds||Cytochrome C|