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Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
Creatinine is a creatine phosphate metabolite that is a result of muscle and protein metabolism. Synthesized by the liver, its presence in the blood can be measured to determine kidney health. Using SIELC’s newly introduced BIST™ method, this creatine metabolite can be retained and separated from urine and plasma on a positively-charged anion-exchange BIST™ B+ column.
There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged analytes to the positively-charged column surface and 2) a mobile phase consisting of a majority of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Creatinine can be separated, retained, and UV detected at 200 nm.
|Column||BIST™ B+, 4.6×150 mm, 5µm, 100A|
|Mobile Phase||MeCN – 90%|
|Buffer||H2SO4 – 0.2%|
|Flow Rate||1.0 ml/min|
|Detection||UV 200 nm|
|Retention time||9.01 min|
|Class of Compounds||Acid|