Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

Creatinine is a creatine phosphate metabolite that is a result of muscle and protein metabolism. Synthesized by the liver, its presence in the blood can be measured to determine kidney health. Using SIELC’s newly introduced BIST™ method, this creatine metabolite can be retained and separated from urine and plasma on a positively-charged anion-exchange BIST™ B+ column. 

There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged analytes to the positively-charged column surface and 2) a mobile phase consisting of a majority of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Creatinine can be separated, retained, and UV detected at 200 nm.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Creatinine

Condition

Column	BIST™ B+, 4.6×150 mm, 5µm, 100A
Mobile Phase	MeCN – 90%
Buffer	H2SO4 – 0.2%
Flow Rate	1.0 ml/min
Detection	UV 200 nm
Retention time	9.01 min

Description

Class of Compounds	Acid
Analyzing Compounds	Creatinine