HPLC Method for Analysis of Colistin A on BIST B+ Column

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

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HPLC Method for Analysis of Colistin A on BIST B+ Column by SIELC Technologies

Colistin A is a last-resort antibiotic drug sued to treat Gram-negative bacterial infection, such as pneumonia. Using SIELC’s newly introduced BIST™ method, Colistin A can be retained on a positively-charged anion-exchange BIST™ B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged Copper and peptide to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Colistin A can be separated, retained, and UV detected at 200 nm

High Performance Liquid Chromatography (HPLC) Method for Analysis of Colistin A

Condition

ColumnBIST™ B+, 4.6×50 mm, 5µm, 100A
Mobile PhaseMeCN – 70%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 200 nm

Description

Class of CompoundsDrugs, Antibiotic
Analyzing CompoundsColistin A