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HPLC Method for Analysis of Colistin A on BIST B+ Column
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
Colistin A is a last-resort antibiotic drug sued to treat Gram-negative bacterial infection, such as pneumonia. Using SIELC’s newly introduced BIST™ method, Colistin A can be retained on a positively-charged anion-exchange BIST™ B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged Copper and peptide to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Colistin A can be separated, retained, and UV detected at 200 nm
High Performance Liquid Chromatography (HPLC) Method for Analysis of Colistin A
Condition
| Column | BIST™ B+, 4.6×50 mm, 5µm, 100A |
| Mobile Phase | MeCN – 70% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
Description
| Class of Compounds | Drugs, Antibiotic |
| Analyzing Compounds | Colistin A |