HPLC Method for Analysis of Chlorpheniramine Maleate on BIST B+ Column

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Chlorpheniramine Maleate on BIST B+ Column by SIELC Technologies

Chlorpheniramine Maleate is a popular antihistamine used to provide relief from the symptoms of allergies, hay fever, and the common cold. Using SIELC’s newly introduced BIST™ method, Chlorpheniramine Maleate, which separates in water, can be retained on a positively-charged anion-exchange BIST™ B column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged analytes to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Chlorpheniramine Maleate can be separated, retained, and UV detected at 210 nm and 275 nm.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Chlorpheniramine Maleate

Condition

ColumnBIST™ B+, 4.6×50 mm, 5µm, 100A
Mobile PhaseMeCN – 85%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 210 nm, 275

Description

Class of CompoundsDrug
Analyzing CompoundsChlorpheniramine Maleate