Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

Chlorpheniramine Maleate is a popular antihistamine used to provide relief from the symptoms of allergies, hay fever, and the common cold. Using SIELC’s newly introduced BIST™ method, Chlorpheniramine Maleate, which separates in water, can be retained on a positively-charged anion-exchange BIST™ B column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged analytes to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Chlorpheniramine Maleate can be separated, retained, and UV detected at 210 nm and 275 nm.

BIST B+ Column 4.6x50mm 5um 100A

High Performance Liquid Chromatography (HPLC) Method for Analysis of Chlorpheniramine Maleate

Condition

Column	BIST™ B+, 4.6×50 mm, 5µm, 100A
Mobile Phase	MeCN – 85%
Buffer	H2SO4 – 0.2%
Flow Rate	1.0 ml/min
Detection	UV 210 nm, 275

Description

Class of Compounds	Drug
Analyzing Compounds	Chlorpheniramine Maleate