HPLC Method for Analysis of Chloroacetic acid, Dichloroacetic acid and Trichloroacetic acid on BIST™ A+ Column

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Chloroacetic acid, Dichloroacetic acid and Trichloroacetic acid on BIST™ A+ Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Chloroacetic acid, Dichloroacetic acid and Trichloroacetic acid

Chloroacetic acid is a popular building block in organic syntheses. Dichloroacetic acid and Trichloroacetic acid are frequently used in cosmetic treatments to remove tattos and in chemical peels, as well as topical medication for the ablation of warts. Using SIELC’s newly introduced BIST™ method, a mixture of these three aicds can be separated on a negatively-charged, cation-exchange BIST™ A+ column, contrary to conventional chromatographic wisdom. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include Calcium acetate and Magnesium acetate. Using this new and unique analysis method, these anions can be separated, retained, and detected with a Conductivity Detector.

Condition

ColumnBIST™ A+, 2.1×100 mm, 3 µm, 100A
Mobile PhaseMeCN – 90%
BufferTMDAP ( N,N,N’,N’-Tetramethyl-1,3-diaminopropane) formate – 5 mM pH 4.0
Flow Rate0.4 ml/min
DetectionConductivity

Description

Class of CompoundsAcid, Carboxylic acid, Haloacetic acid
Analyzing CompoundsChloroacetic acid, Dichloroacetic acid and Trichloroacetic acid