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Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
Bivalirudin is a short, 21-amino-acid peptide. It has 2 basic and 6 acidic groups. It is a naturally-occurring anticoagulant. Asp9-bivalirudin is a process impurity and can be a result of the degradation of the drug bivalirudin. SIELC’s new BIST™ mode can retain and separate Bivalirudin and its degredant with a TMDAP phosphate buffer, and the respective retention times can be controlled by altering the pH of the buffer.
Column | BIST™ A+, 2.1×100 mm, 3µm, 100A |
Mobile Phase | MeCN – 30% |
Buffer | TMDAP phosphate – 10 mM pH 5.0, pH 6.0 |
Flow Rate | 0.2 mL/min |
Detection | UV 275 nm |
Class of Compounds | Drugs, Anticoagulant |
Analyzing Compounds | Bivalirudin and Asp9 – bivalirudin is a process impurity |