Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Arginine, Lysine and Histidine Amino Acids on a BIST™ B+ Column.

Basic amino acids, like Arginine, Lysine, and Histidine, can be difficult to separate and retain in typical reverse-phase chromatography. Arginine is a naturally-occurring, essential amino acid typically found in meat, poultry, and fish. Lysine is another essential amino acid typically found in meat, and also cereal grains. A third essential amino acid, Histidine, is also typtically found in meat, poultry, and also cereal grains (like rice, wheat, and rye). Using SIELC’s newly introduced BIST™ method, these three essential amino acids, which protonate in water, can be retained on a positively-charged anion-exchange BIST™ B column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged amino acid analytes to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Arginine, Lysine, and Histidine can be retained and UV detected at 210 nm.

BIST B+ Column 4.6x150mm 5um 100A

High Performance Liquid Chromatography (HPLC) Method for Analysis of Arginine, Lysine, Histidine

Condition

Column	BIST™ B+, 4.6×150 mm, 5µm, 100A
Mobile Phase	MeCN – 70%
Buffer	H2SO4 – 0.2%
Flow Rate	1.0 ml/min
Detection	UV 210 nm

Description

Class of Compounds	Amino acid
Analyzing Compounds	Arginine, Lysine, Histidine