HPLC Method for Analysis of Acid Red 1 on BIST A Column

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Acid Red 1
High Performance Liquid Chromatography (HPLC) Method for Acid Red 1

Acid Red 1 is a popular red synthetic dye used in a wide variety of food products. Using SIELC’s newly introduced BIST™ method, Acid Red 1, which ionizes in water, can be retained on a negatively-charged, cation-exchange BIST™ A column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged Acid Red 1 analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. The effect of reducing the solvation layer by increasing the organic component concentration in the mobile phase can be clearly seen above. Using this new and unique analysis method, Acid Red 1 can be retained and UV detected at 270 nm.


ColumnBIST™ A, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN – 80/20%
BufferTMDAP formate pH 4.0 – 5,0 mM
Flow Rate1.0 ml/min
DetectionVis 535 nm


Class of CompoundsAcid, Dye
Analyzing CompoundsAcid Red 1