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Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
Guanine (G) is one of the four nucleobases used to form DNA and is a derivative of purine. It pairs with Cytosine (C) in the double helix of DNA (in RNA it is replaced by Uracil, U). Adding a ribose ring to guanine transforms it into the nucleoside Guanosine. Removing a hydroxyl group transforms Guanosine into Deoxyguanosine which is a key building block of DNA. Using SIELC’s newly introduced BIST™ method, Deoxyguanosine, Guanine and Guanosine can be retained and separated on a positively-charged anion-exchange BIST™ B+ column.
There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Phosphoric acid (H3PO4), which acts as a bridge, linking the positively-charged analytes to the positively-charged column surface and 2) a mobile phase consisting of a majority of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Deoxyguanosine, Guanine and Guanosine can be separated, retained, and UV detected at 260 nm.
|Column||BIST™ B+, 4.6×150 mm, 5µm, 100A|
|Mobile Phase||MeCN -85%|
|Flow Rate||1.0 ml/min|
|Detection||UV 260 nm|
|Retention time||9.6, 11.2, 12.8 min|
|Class of Compounds||Nucleosides|
|Analyzing Compounds||Deoxyguanosine, Guanine and Guanosine|