HPLC Method for Analysis of Deoxyguanosine, Guanine and Guanosine on BIST B+

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Deoxyguanosine, Guanine and Guanosine on BIST B+
HPLC Method for Analysis of Deoxyguanosine, Guanine and Guanosine on BIST B+ by SIELC Technologies.

Guanine (G) is one of the four nucleobases used to form DNA and is a derivative of purine. It pairs with Cytosine (C) in the double helix of DNA (in RNA it is replaced by Uracil, U). Adding a ribose ring to guanine transforms it into the nucleoside Guanosine. Removing a hydroxyl group transforms Guanosine into Deoxyguanosine which is a key building block of DNA. Using SIELC’s newly introduced BIST™ method, Deoxyguanosine, Guanine and Guanosine can be retained and separated on a positively-charged anion-exchange BIST™ B+ column. 

There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Phosphoric acid (H3PO4), which acts as a bridge, linking the positively-charged analytes to the positively-charged column surface and 2) a mobile phase consisting of a majority of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Deoxyguanosine, Guanine and Guanosine can be separated, retained, and UV detected at 260 nm.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Deoxyguanosine, Guanine and Guanosine

Condition
ColumnBIST™ B+, 4.6×150 mm, 5µm, 100A
Mobile PhaseMeCN -85%
BufferH3PO4 -0.2%
Flow Rate1.0 ml/min
DetectionUV 260 nm
Retention time9.6, 11.2, 12.8 min
Description
Class of CompoundsNucleosides
Analyzing CompoundsDeoxyguanosine, Guanine and Guanosine