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Category: Dyes

  • HPLC Method for Analysis of  FD&C Yellow 5 (Tartrazine) in M&M’s  on BIST A+ Column

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies High Performance Liquid Chromatography (HPLC) Method for Analysis of  FD&C Yellow 5 (Tartrazine) Tartrazine is a popular, multi-charged yellow synthetic dye used in a multitude of food and cosmetic products, including colorful sugar-coated chocolate candies. Using SIELC’s newly introduced BIST™ method, tartrazine, which dissolves and […]

  • HPLC Method for Analysis of Fast Blue B Salt on BIST B+ Colum

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Fast Blue B Salt is a multicharged basic dye with a variety of applications in chromatographic testing, including in thin layer chromatography and lipase activity testing. Using SIELC’s newly introduced BIST™ method, Fast Blue B Salt can be retained on a positively-charged anion-exchange BIST™ […]

  • HPLC Method for Analysis of DAPI on BIST B+ by SIELC Technologies

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies 4′,6-diamidino-2-phenylindole, also known as DAPI, is a popular fluorescent dye used for DNA staining and fluorescence microscopy. Using SIELC’s newly introduced BIST™ method, DAPI can be retained on a positively-charged anion-exchange BIST™ B+ column.  There are two keys to this retention method: 1) a […]

  • HPLC Method for Analysis of Chicago Sky Blue on BIST A+ Column by SIELC Technologies

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Brilliant Black BN (Food Black 1, E151) and Chicago Skye Blue are two azo dyes with the unique property that they have 4 sulfonate groups. Using SIELC’s newly introduced BIST™ method, Brilliant Black BN and Chicago Sky Blue can be retained and separated into […]

  • HPLC Method for Analysis of Quinoline Yellow WS on BIST A+ Column by SIELC Technologies

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Quinoline Yellow WS (E104, D&C Yellow 10) is a mixture of 3 different derivatives of Quinoline Yellow SS, consisting of monosulfonates, (mostly) disulfonates, and trisulfonates. The dye is typically neon-yellow (yellow with a hint of green). Using SIELC’s newly introduced BIST™ method, Quinoline Yellow […]

  • HPLC Method for Analysis of Brilliant Black BN and Chicago Sky Blue on BIST A Column by SIELC Technologies

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Brilliant Black BN (Food Black 1, E151) and Chicago Skye Blue are two azo dyes with the unique property that they have 4 sulfonate groups. Using SIELC’s newly introduced BIST™ method, Brilliant Black BN and Chicago Sky Blue can be retained and separated into […]

  • HPLC Method for Analysis of Allura Red (FD&C Red 40, E129) on a BIST A Column

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Allura Red (FD&C Red 40,  E129) is a popular red synthetic dye used in a wide variety of food products. Using SIELC’s newly introduced BIST™ method, Allura Red 1, which ionizes in water, can be retained on a negatively-charged, cation-exchange BIST™ A column. There […]

  • HPLC Method for Analysis of Acid Red 1 on BIST A Column

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Acid Red 1 is a popular red synthetic dye used in a wide variety of food products. Using SIELC’s newly introduced BIST™ method, Acid Red 1, which ionizes in water, can be retained on a negatively-charged, cation-exchange BIST™ A column. There are two keys […]

  • HPLC Method for Analysis of Sunset Yellow on BIST A Column

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Sunset Yellow is a popular orange-yellow synthetic dye used in a wide variety of food products. Using SIELC’s newly introduced BIST™ method, Sunset Yellow, which ionizes in water, can be retained on a negatively-charged, cation-exchange BIST™ A column. There are two keys to this […]

  • HPLC Method for Analysis of Tartrazine on BIST A Column

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Tartrazine is a popular yellow synthetic dye used in a multitude of food and cosmetic products. Using SIELC’s newly introduced BIST™ method, tartrazine, which ionizes in water, can be retained on a negatively-charged, cation-exchange BIST™ A column. There are two keys to this retention […]