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Category: Acids

  • HPLC Method for Analysis of Creatinine on BIST B+ Column by SIELC Technologies

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Creatinine is a creatine phosphate metabolite that is a result of muscle and protein metabolism. Synthesized by the liver, its presence in the blood can be measured to determine kidney health. Using SIELC’s newly introduced BIST™ method, this creatine metabolite can be retained and […]

  • HPLC Method for Analysis of Xanthines and Uric Acid on BIST B+ Column by SIELC Technologies

    Separation type: Hydrophilic interaction liquid chromatography (HILIC) Xanthine is a purine base that naturally occurs in the human body, and is a precursor for many other stimulants, medications, and biological compounds, including caffeine and uric acid. Caffeine is one of the most well-known stimulants due to its presence in popular drinks such as coffee, tea, […]

  • HPLC Method for Analysis of Uric Acid on BIST B+ Column by SIELC Technologies

    Separation type: Hydrophilic interaction liquid chromatography (HILIC) Uric acid is the final product of purine metabolism and is commonly found in urine. Using SIELC’s newly introduced BIST™ method, this purine-derivative can be retained on a positively-charged anion-exchange BIST™ B+ column.  There are two keys to this retention method: 1) a multi-charged, negative buffer, such as […]

  • HPLC Method for Analysis of Uric Acid in Urine and Human Serum Samples on BIST B+ Column by SIELC Technologies

    Separation type: Hydrophilic interaction liquid chromatography (HILIC) Uric acid is the final product of purine metabolism and is commonly found in urine. Using SIELC’s newly introduced BIST™ method, this purine metabolite can be retained and separated from urine and plasma on a positively-charged anion-exchange BIST™ B+ column.  There are two keys to this retention method: […]

  • HPLC Method for Analysis of Chlorpheniramine Maleate on BIST B+ Column

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Chlorpheniramine Maleate is a popular antihistamine used to provide relief from the symptoms of allergies, hay fever, and the common cold. Using SIELC’s newly introduced BIST™ method, Chlorpheniramine Maleate, which separates in water, can be retained on a positively-charged anion-exchange BIST™ B column. There […]

  • HPLC Method for Analysis of Succinic Acid on BIST A+ Column

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Methanesulfonic acid is a popular non-volatile catalyst used in organic reactions due to it being a strong acid. Other sulfonic acids, like 1-Pentanesulfonic acid, 1-Heptanesulfonic acid, 1-Decanesulfonic acid, and 1=Dodecanesulfonic acid are typically used in ion chromatography and for organic syntheses. Using SIELC’s newly […]

  • HPLC Method for Analysis of Arginine, Lysine and Histidine Amino Acids on BIST B+

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies HPLC Method for Analysis of Arginine, Lysine and Histidine Amino Acids on a BIST™ B+ Column. Basic amino acids, like Arginine, Lysine, and Histidine, can be difficult to separate and retain in typical reverse-phase chromatography. Arginine is a naturally-occurring, essential amino acid typically found […]

  • HPLC Method for Analysis of Allura Red (FD&C Red 40, E129) on a BIST A Column

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Allura Red (FD&C Red 40,  E129) is a popular red synthetic dye used in a wide variety of food products. Using SIELC’s newly introduced BIST™ method, Allura Red 1, which ionizes in water, can be retained on a negatively-charged, cation-exchange BIST™ A column. There […]

  • HPLC Method for Analysis of Aconitic Acid on BIST A+

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Aconitic acid is a popular compound used to artificial create nutty flavors in foods. It is also a common intermediate in the Kreb’s cycle when Citrate is changed into D-isocitrate. Using SIELC’s newly introduced BIST™ method, Aconitic acidcan be retained easily on a negatively-charged, […]

  • HPLC Method for Analysis of Sulfonic acids on BIST™ A+ 

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Methanesulfonic acid is a popular non-volatile catalyst used in organic reactions due to it being a strong acid. Other sulfonic acids, like 1-Pentanesulfonic acid, 1-Heptanesulfonic acid, 1-Decanesulfonic acid, and 1=Dodecanesulfonic acid are typically used in ion chromatography and for organic syntheses. Using SIELC’s newly […]