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Author: Olga Kolesnik

  • HPLC Method for Separation of of Amines in Non Aqueous MP on BIST B+ Column

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Dopamine is a key neurotransmitter and medical stimulant used to treat low blood pressure, low heart rate, and heart attacks. m-Xylylenediamine (MXDA) is a popular curing agent used on epoxy resins. 2,4,6-Tris(dimethylaminomethyl)phenol is another amine-based curing agent used on epoxy resins. Using SIELC’s newly introduced […]

  • HPLC Method for Analysis of Chromium(III) on BIST B+ Column

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Chromium(III) is a nutrient essential for human metabolism of insulin, sugar, and lipids. Using SIELC’s newly introduced BIST™ method, Cr(III) can be retained on a positively-charged anion-exchange BIST™ B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such […]

  • HPLC Method for Analysis of  FD&C Yellow 5 (Tartrazine) in M&M’s  on BIST A+ Column

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies High Performance Liquid Chromatography (HPLC) Method for Analysis of  FD&C Yellow 5 (Tartrazine) Tartrazine is a popular, multi-charged yellow synthetic dye used in a multitude of food and cosmetic products, including colorful sugar-coated chocolate candies. Using SIELC’s newly introduced BIST™ method, tartrazine, which dissolves and […]

  • HPLC Method for Analysis of Carnosine (beta-alanyl-L-histidine) on BIST B+ Column

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies High Performance Liquid Chromatography (HPLC) Method for Carnosine (beta-alanyl-L-histidine) Condition Column BIST B+, 4.6×50 mm, 5 µm, 100A Mobile Phase MeCN – 75% Buffer H2SO4 – 0.2% Flow Rate 1.0 ml/min Detection UV 205 nm Peak Retention Time 3.71 min Description Class of Compounds Dipeptide […]

  • HPLC Method for Analysis of Fast Blue B Salt on BIST B+ Colum

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Fast Blue B Salt is a multicharged basic dye with a variety of applications in chromatographic testing, including in thin layer chromatography and lipase activity testing. Using SIELC’s newly introduced BIST™ method, Fast Blue B Salt can be retained on a positively-charged anion-exchange BIST™ […]

  • HPLC Method for Analysis of DAPI on BIST B+ by SIELC Technologies

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies 4′,6-diamidino-2-phenylindole, also known as DAPI, is a popular fluorescent dye used for DNA staining and fluorescence microscopy. Using SIELC’s newly introduced BIST™ method, DAPI can be retained on a positively-charged anion-exchange BIST™ B+ column.  There are two keys to this retention method: 1) a […]

  • HPLC Method for Analysis of 3′-Deoxyadenosine and 2′-Deoxyadenosine on BIST B+ Column

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies 3′-Deoxyadenosine (Cordycepin) and 2′-Deoxyadenosine (Deoxyadenosine) are structural isomer derivatives of adenosine. The only structural difference between the two compounds is the replacement of the hydroxyl group (-OH) on either 3’ or 2’ position, respectively. Using SIELC’s newly introduced BIST™ method, 3′-Deoxyadenosine and 2′-Deoxyadenosine can […]

  • HPLC Method for Analysis of Deoxyguanosine, Guanine and Guanosine on BIST B+

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Guanine (G) is one of the four nucleobases used to form DNA and is a derivative of purine. It pairs with Cytosine (C) in the double helix of DNA (in RNA it is replaced by Uracil, U). Adding a ribose ring to guanine transforms […]

  • Ionic Modifier Effect on Selectivity of Separation of Deoxyguanosine, Guanine and Guanosine on BIST B+

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Guanine (G) is one of the four nucleobases used to form DNA and is a derivative of purine. It pairs with Cytosine (C) in the double helix of DNA (in RNA it is replaced by Uracil, U). Adding a ribose ring to guanine transforms […]

  • HPLC Method for Analysis of Chicago Sky Blue on BIST A+ Column by SIELC Technologies

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies Brilliant Black BN (Food Black 1, E151) and Chicago Skye Blue are two azo dyes with the unique property that they have 4 sulfonate groups. Using SIELC’s newly introduced BIST™ method, Brilliant Black BN and Chicago Sky Blue can be retained and separated into […]